pbpC [2017-10-10 11:33:07]

transpeptidase, penicillin-binding protein 3

locus: BSU04140

pI: 6.20

mw: 74.23 kDa

protein length: 668 aa

gene length: 2004 bp

function: unknown

product: penicillin-binding protein 3

essential: no

synonyms: ycsM,yzsA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 463,934 → 465,940

The protein

Catalyzed reaction/ biological activity

• cross-linking of glycan chains [pubmed|28792086]

Protein family

• transpeptidase family (according to Swiss-Prot)

Structure

• [PDB|5E31] (from Enterococcus faecium, 40% identity)

[SW|Localization]

• cell membrane (according to Swiss-Prot)
• mid cell localization (divisome), this depends on [protein|search|FtsZ ]and [protein|EBEFF9E0A524DCDA19382E5401B923B805E4C559|PBP2A ][pubmed|28792086]
• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|pbpC]-[gene|D0A2506FDCEA41D2A8910AC2837B46E0B311D63B|ycsN]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE04140 (Δ[gene|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|pbpC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACTTTCCCCTGCCTTC, downstream forward: _UP4_TAAAATGTCTTTTTAAAAGG
• BKK04140 (Δ[gene|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|pbpC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACTTTCCCCTGCCTTC, downstream forward: _UP4_TAAAATGTCTTTTTAAAAGG

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jeff Errington] lab

References

• 8830698,14731276,20487272,18957862,28792086